发明名称 |
METHODS AND MATERIALS FOR MODULATION OF THE IMMUNOSUPPRESSIVE ACTIVITY AND TOXICITY OF MONOCLONAL ANTIBODIES |
摘要 |
<p>The binding specificity of the murine OKT3 has been transferred into a human antibody framework in order to reduce its immunogenicity. This "humanized" anti-CD3 mAb (gOKT3-5) was previously shown to retain, in vitro, all the properties of native OKT3, including T cell activation which has been correlated, in vivo, with the severe side-effects observed in transplant recipients after the first administration of the mAb. Disclosed is a single amino acid mutation from a leucine to a glutamic acid at position 235 in the Fc receptor (FcR) binding segment of the gOKT3-5 mAb to produce Glu-235 mAb. Also disclosed is an amino acid mutation from the contiguous phenylalanine at position 234 to a leucine (Leu-234).</p> |
申请公布号 |
EP0700402(B1) |
申请公布日期 |
2002.11.27 |
申请号 |
EP19940920062 |
申请日期 |
1994.06.01 |
申请人 |
TOLERANCE THERAPEUTICS, INC. |
发明人 |
BLUESTONE, JEFFREY, A.;ZIVIN, ROBERT, A.;JOLLIFFE, LINDA |
分类号 |
C12N15/02;A61K38/00;A61K39/395;A61P37/02;A61P37/06;C07K16/24;C07K16/28;C07K16/46;C12N15/09;C12P21/08;C12R1/91;(IPC1-7):A61K39/395 |
主分类号 |
C12N15/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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