Benign method using electromagnetic fields to improve cardiovascular cytoprotection

摘要

A unique approach to clinical application of cytoprotection is offered by electromagnetic (EM) field induction of stress proteins. Electromagnetic fields are non-invasive and easily applied, compared to the current hyperthermia protocols. Fertilized dipteran eggs and cultured rodent cardiomyocytes (H9c2 cells) were used as first-level models to test electromagnetic fields for their ability to induce increased hsp70 levels for effective cytoprotection. Eggs preconditioned with an 8 muT 60 Hz EM field for 30 minutes had a 114% increase in hsp70 levels, and an average 82% increase in survival following a lethal temperature of 36.5° C. Thermal pre-conditioning at 32° C. was not nearly as effective in dipteran eggs, inducing only a 44% increase in survival. Preconditioning of cultured murine cardiomyocytes (H9c2 cells) with an 8 muT 60 Hz field induced a 77% average increase in hsp70 levels.