NEW GENE HAVING DNA SEQUENCE ENCODING HYDROXYNITRILELYASE, RECOMBINANT PROTEIN DERIVED FROM THE SAME AND HAVING HYDROXYNITRILELYASE ACTIVITY, AND USE THEREOF

摘要

PROBLEM TO BE SOLVED: To establish a base capable of providing R-hydroxynitrilelyase in an amount necessary for industrial application. SOLUTION: A gene encoding a hydroxynitrilelyase is cloned by a primer based on a DNA sequence of 5' region of md1 gene derived from Prunus serotina and Prunus amygdalus and/or based on 3' region of one DNA sequence of a hydroxynitrilelyase isozyme. Moreover, a recombinant protein capable of being produced in a suitable host cell by foreign expression of a DNA sequence of HNL gene, a protein induced therefrom and an induced protein, and use of the proteins aiming at production of (R)- or (S)-cyanohydrin are disclosed.