发明名称 |
Method for measuring antigen concentration |
摘要 |
This invention provides a method for measuring an antigen concentration in a sample, which comprises: preparing VH-domain polypeptide and VL-domain polypeptide of an antibody specific to the antigen; labeling one of the polypeptides with a reporter molecule to form labeled polypeptides, and immobilizing the other polypeptide onto solid-phase to form immobilized polypeptides; contacting the antigen-containing sample and the labeled polypeptides with the solid-phase; and measuring the reporter molecule of the labeled polypeptides bound to the immobilized polypeptides. The present invention permits simpler and quicker sandwich ELISA for measurements of an antigen concentration in high sensitivity.
|
申请公布号 |
US6482596(B1) |
申请公布日期 |
2002.11.19 |
申请号 |
US19990313350 |
申请日期 |
1999.05.18 |
申请人 |
ROCHE DIAGNOSTICS CORPORATION |
发明人 |
UEDA HIROSHI;NAGAMUNE TERUYUKI;NISHIMURA HAJIME;KUMAGAI IZUMI;TSUMOTO KOUHEI;MAHONEY WALTER C.;WINTER GREG;SCHUELER PAULA A. |
分类号 |
C07K16/18;G01N33/68;(IPC1-7):G01N33/53;G01N33/543;G01N33/573 |
主分类号 |
C07K16/18 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|