REAGENTS AND METHODS FOR THE QUANTIFICATION OF TOTAL DOXEPINS IN BIOLOGICAL FLUIDS

摘要

Immunoassay methods and reagents for the quantification of total doxepins (i.e., E-doxepin, Z-doxepin, E-desmethyldox- epin, and Z-desmethyldoxepin) in a test sample are disclosed. The quantification of total doxepins is accomplished in an immu- noassay employing antibodies and labeled reagents prepared with doxepin derivatives of formula (II), wherein Y-Z can be C=CH or N-CH2, R1 is a linking group, R2 can be H or CH3 and Q can be a detectable moiety or an immunogenic carrier material. The antibody reagent comprises antibodies which are capable of binding to total doxepins and which are produced with one or more immunogens prepared from the doxepin derivative of formula (II), and the labeled reagent is also pre- pared from the doxepin derivative of formula (II).