| 摘要 |
PROBLEM TO BE SOLVED: To provide an improved vector capable of synthesizing RNA fraction largely deleting extra sequences in vitro. SOLUTION: This vector has a DNA cloning site within 50 bp of the downstream of a promoter sequence of a RNA polymerase and at least one breakage site of the vector in the downstream of the cloning site and the DNA cloning site has a blunt end type restriction enzyme recognition sequence and the breakage site of the vector has a blunt end type or 5'-protruding end type restriction enzyme recognition sequence. |
