发明名称 |
Biosensor device and method |
摘要 |
A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an alpha-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
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申请公布号 |
US6478939(B1) |
申请公布日期 |
2002.11.12 |
申请号 |
US20000578353 |
申请日期 |
2000.05.24 |
申请人 |
PENCE, INC.;MCGILL UNIVERSITY |
发明人 |
LENNOX R. BRUCE;HODGES ROBERT S.;IRVIN RANDALL T. |
分类号 |
C12Q1/00;C12Q1/68;C40B40/10;G01N27/414;G01N33/543;(IPC1-7):G01N27/26;G01N21/00 |
主分类号 |
C12Q1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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