Culturing different cell populations on a decellularized natural biostructure for organ reconstruction

摘要

artificial organs are reconstructed using a three-dimensional scaffold produced by decellularizing biostructures from a donor organ. The three-dimensional scaffold is perfused with isolated endothelial cells that develop to produce an endothelial tissue layer with a primitive vascular system that sustains the growth and development of a second cultured cell population. When grown in the three-dimensional scaffold containing the endothelial tissue layer, with the primitive vascular system, the cells of the second cell population proliferate, mature and differentiate into neomorphic organ structures that are analogous to their in vivo counterparts.