IN VIVO METHOD FOR MEASURING BINDING OF CHEMICAL ACTIVES TO SKIN OR SPECIFIC CONSTITUENTS OF SKIN

摘要

An in vivo method for measuring the binding of chemical compounds or mixtures of compounds to skin constituents comprising: (a) choosing a desired site of any desired size on a subject's body; (b) exposing said site, the binding of which is to be measured, to the chemical compound or mixture of compounds by any desirable method; (c) applying a luminescent or optically absorbing probe/marker to the area that was exposed to the chemical compound or compounds; (d) measuring the luminescence or absorbance spectrum of the probe/marker on skin; and (e) determining the binding of the chemical compound or compounds the body site was exposed to from the luminescence or absorbance spectrum.