RECOMBINANT DIMORPHIC FUNGAL CELL

摘要

It is an object of the present invention to provide fungal host organisms capable of eypressing recombinant proteins while at the same time exhibiting satisfactory growth characteristics. It is a further object to provide - in a single fungal host organism - the characteristic of homogenous growth and low vicosity typically assocated with yeat organisms, and the capability for high protein secretion normally associated wiht famentous fungi. It is a jet further object of the inventio to provide useful tools for genetic anlysis in zygomycetes, inclding dimorphic zygomycetes. Accordingly, the present invention relates to a recombinant, fungal cell or dimorphic fungal cell comprising a regulatable expression of a regulator of morphology. The neuleotide sequence encoding the al least on regulator of morphology is operably linked to an eypression signal not natively associated therewith. Expression of the at least one regulator of morpology directed by the eypression signal not natively associated therewith results in a dimorphic shift of dimorphic fungal cell or a desirable, improved filamentation of a fungal cell or a dimorphic fungal cell. The improved filamenetatio of the fungal cell or the dimorphic fungal cell is positively correlated wiht an increased production and/or secretion of desirable polypeptide.