| 摘要 |
Methods for modulating the hyprocretin system, as well as method for identifying compounds that act as hyprocretin-system modulators are provided. In modulating the hyprocretin system, the method comprises adminstering a therapeutically effective amount of a preprohyprocretin-expression modulator to an individual, wherein the preprohyprocretin-expression modulator alters preprohypocretin expression in preprohypocretin-expressing cells. The method for identifying compounds comprises contacting a test compound to cells equipped with the 5' flanking promoter of the preprohypocretin gene operably linked to a nucleic acid sequence and determining whether the test compound alters transcription of the nucleic acid sequence in the cell, wherein the test compound's ability to alter transcription is indicative of a compound that modulates the hypocretin system. The invention also provides compounds, pharmaceutical compositions, nucleic acid sequences, expression vectors, transformed host cells, and the like for carrying out the methods. |
