发明名称 |
Method of determining gene copy number |
摘要 |
A method for quantitatively determining the relative copy number of a gene of interest introduced recombinantly into a cell is disclosed. The method includes the steps of propagating, in a suitable culture medium, cells that are transformed with a chimeric gene construct containing and capable of expressing the gene introduced into the cells, where said construct includes (i) a control nucleic acid segment having a region of DNA capable of hybridizing with a region of native genomic DNA associated with the cell; and (ii) a second nucleic acid region that contains said gene of interest; isolating DNA from the cells; and determining the ratio of the control nucleic acid segment to the region of native genomic DNA in the isolated DNA as a quantitative measure of the relative copy number of the gene in the cell.
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申请公布号 |
US2002164613(A1) |
申请公布日期 |
2002.11.07 |
申请号 |
US20010010071 |
申请日期 |
2001.11.06 |
申请人 |
VILLARETE LORELIE H.;GHUMAN MANDEEP;LI WAYNE W. |
发明人 |
VILLARETE LORELIE H.;GHUMAN MANDEEP;LI WAYNE W. |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68;C12P19/34;C12N15/87 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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