METHOD FOR THE STABLE INVERSION OF DNA SEQUENCE BY SITE-SPECIFIC RECOMBINATION AND DNA VECTORS AND TRANSGENIC CELLS THEREOF
摘要
The invention relates to a method for the stable inversion of a DNA fragment upon recombinase-mediated rearrangements using two sets of two incompatible site-specific recombinase targeting sites (SSRTS) in the same order but in reverse orientation flanking said DNA fragment to be inverted. The invention also relates to a method for the stable inversion of said DNA fragment upon rearrangement mediated by a recombinase such as Cre recombinase. The invention also relates to a method for obtaining a transgenic cell of which at least one allele of a DNA sequence of interest is invalidated by a process of conditional deletion and the genome of which comprises a reporter gene inserted at the place of the DNA fragment deleted by said process of conditional deletion. The invention also concerns a method to generate targeting sites to perform site-specific recombination mediated cassette exchange. The corresponding vectors, host cells, and transgenic animals are claimed.
申请公布号
WO02088353(A2)
申请公布日期
2002.11.07
申请号
WO2002IB02493
申请日期
2002.04.19
申请人
ASSOCIATION POUR LE DEVELOPPEMENT DE LA RECHERCHE;CHAMBON, PIERRE;GHYSELINCK, NORBERT, B.;SCHNUETGEN, FRANK
发明人
CHAMBON, PIERRE;GHYSELINCK, NORBERT, B.;SCHNUETGEN, FRANK