EFFICIENT PRODUCTION OF MULTIPLE SHOOTS FROM THIDIAZURON-TREATED MATURE EMBRYOS AND LEAF-BASE/APICAL MERISTEMS OF BARLEY (HORDEUM VULGARE L.) GENOTYPES

摘要

The in vitro plant regeneration frequencies for immature scutella, leaf- bases/apical meristems (LB/AM) and mature embryos of four commercially important barley genotypes were compared. Production of shoots from mature embryos or calli o f LB/AM incubated on media containing 1.0 or 2.0 mg/L 6-benzyiaminopurine (BA P) were comparable to regeneration frequencies obtained for scutella-derived calli of the same genotypes. Incubation of excised mature embryos and LB/AM on media containing an alternative plant growth regulator, thidiazuron (TDZ), resulte d in an increased shoot production. However, TDZ treatment did not stimulate plant regeneration from calli derived from scutella or LB/AM. Shoots formed from T DZ- treated mature embryos and LB/AM were induced without a callus interphase an d the in vitro culture system gave a three- to sight-fold higher regeneration frequency than recorded for scutella-derived calli on BAP medium. The simplicity and rapid development of shoots using the mature embryo system strongly favours its us e over commonly used regeneration systems.