METHODS AND COMPOSITION FOR THE PRODUCTION OF ORTHOGONAL TRNA-AMINOACYL TRNA SYNTHETASE PAIRS

摘要

This invention provides compositions and methods for generating components o f protein biosynthetic machinery including orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases. Metho ds for identifying orthogonal pairs are also provided. These components can be used to incorporate unnatural amino acids into proteins in vivo.