发明名称 |
Method for producing recombinant insulin from fusion proteins. |
摘要 |
This invention relates to a DNA encoding a fusion protein of formula (I) ÄYÜ-ÄX1Ü-ÄB-chainÜ-ÄX2Ü-ÄLinkerÜ-ÄX3Ü-ÄA-chainÜ wherein Y is a leader peptide sequence for the expression and secretion of the protein, comprising at least one amino acid residue; X1 is an amino acid sequence which is cleavable enzymatically or chemically; B-chain is the amino acid sequence of insulin B chain; X2 is an amino acid sequence which is cleavable enzymatically; Linker is a linker sequence comprising at least one amino acid residue; X3 is an amino acid sequence which is cleavable enzymatically; and A- chain is the amino acid sequence of insulin A chain, and to a method for producing insulin using an expression system containing the DNA in high efficiency and high yield. |
申请公布号 |
ZA200108951(B) |
申请公布日期 |
2002.10.30 |
申请号 |
ZA20010008951 |
申请日期 |
2001.10.30 |
申请人 |
ITOHAM FOODS INC. |
发明人 |
SHUSAKU OKA;SEIJI SATO;NAOHIKO HIGASHIKUNI;MASAAKI KONDO;TOSHIYUKI KUDO;SHIGEAKI WATANABE;YOSHIHIRO WAKI;HIROTAKA YUKI |
分类号 |
C12N15/09;C07K14/575;C07K14/62;C07K19/00;C12N1/21;C12N15/00;C12N15/17;C12P21/02;C12R1/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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