PLANT ADPGLUCOSE PYROPHOSPHATASE AND ITS USE IN THE PRODUCTION OF ASSAY DEVICES

摘要

Plant ADPglusose pyrophosphatases, process for production thereof, use in the production of assay devices and in obtaining transgenic plants. AGPPase is an enzyme that catalyses the hydrolysis of a wide range of small molecules with phosphodiester bonds, of which ADPG (adenosine diphosphate glucose) stands out. The enzyme obtained from plant extracts is used in assay devices for determining nucleoside diphosphate sugar levels, based either in the sugar-1-phosphate released, or in the monophosphate nucleoside products, both formed by the reaction catalysed by AGPPase, as well as the detection of sulphonucleotides such as 3'-phosphoadenosine 5'phosphosulphate (PAPS) and adenosine 5'phosphosulphate (APS). Similarly, partial sequences are described of amino acids of the enzyme, its cDNA and the protein deduced. Also described are the steps of purification and the characterisation of a new isoform of ADPglucose pyrophosphatase that is associated with the starch granules in plants. Finally, the production of transgenic plants is described that over-express AGPPase and which possess a high content of sugars and a high resistance to high salt concentrations.