发明名称 |
Type 1 and type p fimbriae-adhesins isolated from novel e. coli strains, process for their preparation and uses thereof |
摘要 |
Fimbriae adhesins have a molecular weight of 2x105 and 2x107 Da, and are comprised of 90-95% protein and 1-3% sugar. Type 1 fimbriae include five different protein fractions of 14-20 kDa, most of which are associated with carbohydrates. Type P fimbriae also include five different protein fractions of 14-20 kDa, and one of the majority proteins is associated with carbohydrates. The process of the invention comprises: culturing E. coli strains CECT 4484 and CECT 4485; collecting the sediment by centrifugation and resuspending it in physiological saline followed by homogenization; centrifuging the homogenate and collecting the supernatant; precipitating the supernatant with saline, reconstituting the precipitate and dialyzing the solution; treating the dialyzate with sodium deoxycholate and, subjecting the product to two successive chromatographies with Sephacryl S-200 and Sepharose 4B. The product is used for treatment and prevention of infections of the urinary tract caused by fimbriated E. coli.
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申请公布号 |
US6471966(B1) |
申请公布日期 |
2002.10.29 |
申请号 |
US19980128484 |
申请日期 |
1998.08.04 |
申请人 |
INDUSTRIAL FARMACEUTICA Y DE ESPECIALIDADES, S.A. |
发明人 |
PELAEZ RICARDO PALACIOS;GARATE ALBERTO MARTINEZ;QUESADA JORGE MARTINEZ |
分类号 |
A61K38/00;A61K39/00;C07K14/245;(IPC1-7):A61K39/02;A61K45/00;C12N1/00;C12N1/20 |
主分类号 |
A61K38/00 |
代理机构 |
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