Flow cell and method of operating therefor

摘要

A flow cell for analysis of particles in a sample fluid buffered by sheath fluid has a sample inlet to receive the sample fluid and a sheath inlet for receiving the sheath fluid. The sample fluid flows in a flow direction from the sample inlet past an examination region to an outlet. A flow distributor is positioned between the sheath inlet and the examination region for distributing the sheath fluid ranging in a first direction substantially paralleled to the flow direction to the second direction substantially perpendicular to the flow direction. The distribution of the sheath fluid at the output of the distributor is vortex-free over a wide range of input directions and input chamber geometries. It eliminates the creation of vortices that could cause mixing and instability of the sample fluid in the examination area and allows greater freedom in shaping the fluid system for optimal hydrodynamic focusing.