Method for determining antibiotics containing a beta-lactam ring in a biological fluid

摘要

A method for detecting antibiotics with -lactam core in a biological fluid comprising the following steps: a) contacting a specific volume of said biological fluid with an amount of identifying agent and incubating the resulting mixture in conditions for forming a complex of the antibiotics possibly present in said biological fluid with the identifying agent; b) contacting the mixture resulting from step a) with at least a reference antibiotic immobilised on a support, in conditions for forming a complex of the reference antibiotic with the amount of identifying agent which has not reacted in step a); and c) determining the amount of identifying agent fixed on the support. The recognition agent comprises a receptor sensitive to antibiotics with a -lactam core obtained from Bacillus licheniformis and is selected from BlaR receptor and BlaR-CTD receptor.