METHODS USING O6-ALKYLGUANINE-DNA ALKYLTRANSFERASES

摘要

A method using O6-alkylguanine-DNA alkyltransferases (AGT) is disclosed for transferring a label from a substrate to a fusion protein comprising the AGT . This allows the detection and/or manipulating of the fusion protein, both in vitro and in vivo, by attaching molecules to the fusion proteins that introduce a new physical or chemical property to the fusion protein. Example s of such molecules are, among others, spectroscopic probes or reporter molecules, affinity tags, molecules generating reactive radicals, cross- linkers, ligands mediating protein-protein interactions or molecules suitabl e for the immobilisation of the fusion protein.