| 发明名称 |
MUTANTS OF DESOXYCYTIDINE KINASE HAVING EXTENDED ENZYMATIC ACTIVITY |
| 摘要 |
The invention relates to a method for artificial in vivo evolution of proteins, said method making it possible to bring about the evolution of a protein X by complementation of a relative protein Y,X and Y both belonging to the same class of enzyme commission (EC) nomenclature or belonging to related classes. The mutants D133E and R104Q of desoxycytidine kinase (DCK) were obtained; both of said mutations result in acquisition of thymidine kinase activity by DCK. |
| 申请公布号 |
WO02083892(A2) |
申请公布日期 |
2002.10.24 |
| 申请号 |
WO2002FR01252 |
申请日期 |
2002.04.10 |
| 申请人 |
INSTITUT PASTEUR;CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);MARLIERE, PHILIPPE;POCHET, SYLVIE;BOUZON, MADELEINE |
发明人 |
MARLIERE, PHILIPPE;POCHET, SYLVIE;BOUZON, MADELEINE |
| 分类号 |
C12N15/09;A61K48/00;A61P43/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/10;C12N9/12;C12N15/01;C12N15/10;C12N15/54 |
主分类号 |
C12N15/09 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
|
| 地址 |
|
