Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficinecy and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch reparir activity to the bacteria.
申请公布号
WO0159092(A9)
申请公布日期
2002.10.24
申请号
WO2001US04339
申请日期
2001.02.12
申请人
THE JOHNS HOPKINS UNIVERSITY;NICOLAIDES, NICHOLAS, C.;SASS, PHILIP, M.;GRASSO, LUIGI;VOGELSTEIN, BERT;KINZLER, KENNETH, W.
发明人
NICOLAIDES, NICHOLAS, C.;SASS, PHILIP, M.;GRASSO, LUIGI;VOGELSTEIN, BERT;KINZLER, KENNETH, W.
