Process for the preparation of conjugated estrogens from pregnant mare urine

摘要

This invention is directed to a process for the preparation of conjugated estrogens from pregnant mare urine (PMU), comprising the steps of (a) treating PMU material with an alkaline solvent; (b) filtrating the treated PMU material to remove mucilaginous substances and solids; (c) contracting the filtered PMU resultant with a sufficient quantity of polystyrene absorber resins; and (d) eluting the resins with a water-miscible solvent to separate conjugate estrogens and resins and then obtaining crude extract conjugated estrogens.