| 摘要 |
<p>This invention provides a method of determining an amino acid sequence of a parent polypeptide. The method consists of: (a) obtaining mass spectra of two or more differentially labeled polypeptide fragments of a parent polypeptide; (b) assigning a mass and a weighting characteristic to two or more paired signals having mass difference; (c) selecting from the mass spectra a paired signal having the assigned mass and a weighting characteristic distinguishable from non-peptide signals; (d) determining the difference in mass of the polypeptide fragments; (e) assigning the mass differences; and (f) orienting the assigned amino acid names. Also, provided is a method of determining the amino acid sequence of a polypeptide consisting of: (a) constructing a graph from mass spectra of two or more differentially labeled polypeptides; (b) creating a node corresponding to a paired signal; and (c) adding a labeled weighted directed edge to the graph between any two nodes.</p> |
