| 摘要 |
The instant invention provides methods and systems for searching genomic databases using polypeptide sequence information, such as those obtained fro m peptide sequencing projects, especially those using mass spectrometers. According to the instant invention, polypeptide sequences can be reverse translated into multiple sequence tags wich are then used to search for identical or similar sequences in genomic databases, such as unanotated genomic databases of human or other organisms. Alternatively, the polypeptid e sequences can be directly compared to sequences translated from at least 3, preferably all 6 reading frames of genomic sequences. The instant invention also provides systems for performing the methods of the instant invention, including computer systems, and systems including said computer systems and mass spectrometers linked to said computer systems. The instant invention further provides methods of conducting proteomic businesses using the method s of the instant invention.
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