发明名称 |
Polynucleotide sequence assay |
摘要 |
Disclosed are methods for detecting or quantifying one or more target polynucleotide sequences in a sample. In one aspect, a sample is contacted with first and second probe pair that are capable of hybridizing to a selected target sequence and a corresponding complementary sequence, respectively. Probe cleavage and ligation results in the formation of ligation products which can be generated in an exponential fashion when the target sequence and/or complement are present in the sample. In another embodiment, a single probe pair can be used to form ligation product in a linear fashion from a complementary template. Reagents and kits are also disclosed.
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申请公布号 |
US2002150904(A1) |
申请公布日期 |
2002.10.17 |
申请号 |
US20010898323 |
申请日期 |
2001.07.03 |
申请人 |
BI WANLI;LIVAK KENNETH J.;BLOCH WILL |
发明人 |
BI WANLI;LIVAK KENNETH J.;BLOCH WILL |
分类号 |
G01N27/62;C12N15/09;C12Q1/68;G01N21/64;G01N21/76;G01N27/447;G01N33/53;G01N33/566;G01N33/68;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
G01N27/62 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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