UNCOUPLED PRODUCTIVE AND CATABOLIC HOST CELL PATHWAYS

摘要

The invention provides methods for producing products comprising improved ho st cells genetically engineered to have uncoupled productive and catabolic pathways. In particular, the present invention provides host cells having a modification in nucleic acid encoding an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a modification of nucleic acid encoding an enzymatic activity that phosphorylates D-gluconate at its 6 th carbon. Such improved host cells are used for the production of products, su ch as, ascorbic acid intermediates. Methods for making and using the improved host cells are provided. Nucleic acid and amino acid sequences for glucokina se and gluconokinase are provided.