发明名称 |
Homologous recombination in mismatch repair inactivated eukaryotic cells |
摘要 |
A mammalian cell having a mismatch repair-deficient phenotype is provided, where one or both alleles of a gene essential for mismatch repair, such as an Msh gene, are inactivated. Using this cell in a gene knock-out methodology advantageously allows efficient homologous recombination, even when the DNA sequences of the donor and recipient sequences diverge by significantly more than 0.6%.
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申请公布号 |
US2002151059(A1) |
申请公布日期 |
2002.10.17 |
申请号 |
US20010884877 |
申请日期 |
2001.06.20 |
申请人 |
TE RIELE HENRICUS PETRUS JOSEPH;DE WIND NIELS;DEKKER-VLAAR HELENA MARIA JOHANNA |
发明人 |
TE RIELE HENRICUS PETRUS JOSEPH;DE WIND NIELS;DEKKER-VLAAR HELENA MARIA JOHANNA |
分类号 |
C12N15/85;C12N15/90;(IPC1-7):C12N15/87 |
主分类号 |
C12N15/85 |
代理机构 |
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