发明名称 |
Polynucleotide pools enriched in either high-abundance or low-abundance sequences |
摘要 |
This invention provides novel methods for producing a plurality of polynucleotides prepared from a polynucleotide sample and the plurality of polynucleotides so produced. The plurality of polynucleotides is either substantially enriched in high-abundance polynucleotide sequences relative to the polynucleotide sample or substantially enriched in low-abundance polynucleotide sequences relative to the polynucleotide sample. Each of the polynucleotides in the plurality of polynucleotides includes a RNA promoter sequence and a universal primer site. The invention also provides kits useful in the methods of the invention and for using the pools of polynucleotides produced thereby. The polynucleotide pools are useful in a wide variety of applications, such as cloning, expression, and hybridization studies. Pools enriched in low-abundance polynucleotides are particularly useful in studies aimed at identifying important regulatory proteins.
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申请公布号 |
US6465219(B1) |
申请公布日期 |
2002.10.15 |
申请号 |
US20000632898 |
申请日期 |
2000.08.07 |
申请人 |
GENEMED BIOTECHNOLOGIES, INC. |
发明人 |
ZHU YORK YUAN-YUAN |
分类号 |
C12Q1/68;(IPC1-7):C12P19/34;C07H21/04 |
主分类号 |
C12Q1/68 |
代理机构 |
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地址 |
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