REVERSIBLE IMMORTALIZATION

摘要

A gene complex for reversible immortalization of cells contains an immortalizing gene domain including at least one resistance gene, an immortalization gene and preferably a suicide gene, in addition to sequences flanking said gene domain and acting as a recognition point for homologous, intermolecular recombination, as well as at least one promoter (fig. 1) whic h prefixes the gene domain. A gene complex for immunomodulation of cells contains a first immunomodulated gene domain whose expression inhibits the function of MHC I molecules, a second immunomodulating gene domain whose expression results in an inactivation of natural killer cells, and a resistance gene. Organ-related cells are provided according to a method for obtaining cells; said cells are immortalized by transferring the first gene complex and are immunomodulated by transferring the second gene complex. The immortalization is reversed after expansion of the immortalized cells has occurred.