| 摘要 |
<p>A new method for an antibiotic-free selection of genetically modified cells is described. It is shown that antisense molecules targeted to an essential cellular gene inhibits growth and is suited as an agent for growth selection of cells transformed with a plasmid carrying an altered version of the essential gene. The results show that antisense molecules may be used for antibiotic-free selection of desired transformed microbes when targeted against an essential microbial gene. This technology is useful in genetic engineering for research growth and isolation of transformed organisms, and for industrial growth maintenance of transformed organisms, e.g. in the production of genetically engineered proteins as an environmentally safer alternative to traditional selection methods based on antibiotics.</p> |
