NOVEL NUCLEIC ACID PROBE AND NOVEL METHOD OF ASSAYING NUCLEIC ACIDS USING THE SAME

摘要

A novel nucleic acid probe for simultaneously assaying nucleic acids with a simple apparatus in case where one or more nucleic acids are present in the assay system, characterized in that, in a single-stranded nucleic acid probe labeled with a plural number of fluorescent pigments so as to form at least one pair of fluorescent pigments causing the FRET phenomenon (i.e., a fluorescent pigment capable of serving as a donor pigment and another fluorescent pigment capable of serving as an acceptor pigment), the base sequence is designed so that the fluorescent intensity of the acceptor pigment is weakened when the probe is hybridized with the target nucleic acids, and the above pigments are labeled.