Episomally replicating vector, its preparation and use

摘要

The present invention relates to stably episomally replicating vectors, comprising at least one scaffold/matrix attached region (S/MAR) which binds to nuclear matrix proteins that contain a SAF-A consensus sequence, at least one viral or eukaryotic origin of replication (ORI), at least one transcription unit transcribed in direction towards the S/MAR, and a polyadonylation signal within the S/MAR or in transriptional direction after the S/MAR, cells comprising these, processes for their preparation, and their use, in particular as a medicament or diagnostic.