PROBE FOR VISUALIZING PHOSPHORYLATION/DEPHOSPHORYLATION OF PROTEIN AND METHOD OF DETECTING AND QUANTIFYING PHOSPHORYLATION/DEPHOSPHORYLATION OF PROTEIN

摘要

A widely applicable method of detecting and quantifying the phosphorylation/dephosphorylation of a protein in cells whereby the phosphorylation/dephosphorylation of a protein in vital cells, animals, plants, etc. can be non-destructively monitored and spatial and time-course analyses can be performed. In this method, use is made of a probe for visualizing phosphorylation/dephosphorylation characterized in that a tandem fusion unit, which has a substrate domain having a phosphorylation/dephosphorylation site and a phosphorylation-recognizing domain attached thereto via a linker sequence, is located between a donor chromophore and an acceptor chromophore in fluorescence resonance energy transfer.