发明名称 |
Novel thermostable phytase with 2.1Åcrystal structure |
摘要 |
The present invention relates to a thermostable enzyme with a 2.1 crystal structure of a propeller type comprising six external blades that encompass the outer boundary of the crystal and six internal calcium-binding sites that are embedded inside the above crystal. Each of the six blades comprises 4 or 5 anti-parallel beta-strands, and the six calcium binding sites consist of 3 high-affinity calcium binding sites and 3 low-affinity binding sites, which are involved in the enzyme's thermostability and catalytic activity, respectively. The above-mentioned Ca2+ binding motifs are expected to be utilized in synthesizing highly thermostable proteins and the elucidation of active sites of an enzyme from a three-dimensional structure can help to design new enzymes having those sites with the aid of recent advanced technology of protein engineering
|
申请公布号 |
US2002142433(A1) |
申请公布日期 |
2002.10.03 |
申请号 |
US20020043623 |
申请日期 |
2002.01.09 |
申请人 |
OH TAE KWANG;OH BYUNG CHUL;HA NAM CHUL;CHOI YANG WOONG;LEE DONG KYU;OH BYUNG HA |
发明人 |
OH TAE KWANG;OH BYUNG CHUL;HA NAM CHUL;CHOI YANG WOONG;LEE DONG KYU;OH BYUNG HA |
分类号 |
C12N9/00;C12N9/16;(IPC1-7):C12N9/14 |
主分类号 |
C12N9/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|