发明名称 |
METHOD FOR DETECTING MYCOPLASMA GENITALIUM |
摘要 |
PROBLEM TO BE SOLVED: To provide a method for detecting Mycoplasma genitalium. SOLUTION: This is a method for detecting Mycoplasma genitalium by carrying out real-time PCR by using a DNA obtained from a specimen as a template and using a probe, wherein the probe used in the real-time PCR is designed based on the base sequence of the region corresponding to base no.610-644 or 792-869 of a base sequence represented by sequence no.1 (refer to specification) of 16S RRNA gene, and a primer pair used in the real-time PCR is designed based on a base sequence conserved at least in M. genitalium in the region corresponding to base no.497-1051 of a base sequence represented by sequence no.1 of 16S rRNA gene and so as to permit amplifying a region containing the base sequence of the probe. |
申请公布号 |
JP2002281980(A) |
申请公布日期 |
2002.10.02 |
申请号 |
JP20010092994 |
申请日期 |
2001.03.28 |
申请人 |
MITSUBISHI KAGAKU BIO-CLINICAL LABORATORIES INC |
发明人 |
ISHIKO HIROAKI;YOSHIDA TAKASHI |
分类号 |
C12N15/09;C12Q1/68;C12R1/35 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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