RECOMBINANT PLASMID DNA P7SHSHIS FOR PRODUCTION OF DIMERIC SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF ESCHERICHIA COLI AS PRODUCER OF DIMERIC FORM OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS

摘要

biotechnology, genetic and protein engineering. SUBSTANCE: invention relates to the constructed recombinant plasmid DNA p7SHSHis for production of dimeric single-stranded antibodies of tick-borne encephalitis virus of molecular mass 2.5 Md. The strain of bacterium E. coli B-774 is transformed with recombinant plasmid p7SHSHis. Firstly obtained plasmid DNA and the bacterial strain-producer provide expression of dimeric form of single-stranded antibodies of tick-borne encephalitis virus retaining the conformation of variable domain of the parent monoclone antibodies. EFFECT: plasmid and strain-producer indicated above, valuable biological properties. 2 cl, 7 dwg