Method for recovering proteins from the interstitial fluid of plant tissues

摘要

A method for large-scale recovery of a concentrated and active protein of interest such as an enzyme from the interstitial fluid of plant tissues comprises: (a) infiltrating a plant tissue with a buffer solution such as citrate, phosphate or Tris; (b) subjecting the plant tissue and buffer solution to a substantially vacuum environment; (c) removing the tissue from the buffer solution; (d) centrifuging the tissue to remove interstitial fluid; and (e) concentrating the protein of interest from the interstitial fluid.