| 摘要 |
Expression systems comprising chimeric promoters with binding sites for recombinant transcription factors The present invention relates to a nucleic acid construct which comprises the following components: Component a): at least one promoter Component b): a nucleic acid sequence encoding at least one recombinant transactivator whose transcription is activated by component a) and which comprises: component b1): a nucleic acid sequence encoding a DNA-binding domain component b2): a nucleic acid sequence encoding a transactivation domain comprising glutamine, serine and threonine Component c): at least one a nucleic acid sequence sequence for binding the expression product of component b) Component d): at least one promoter which comprises the CDE-CHR element or the E2FBS-CHR element and whose 5' end is bound, i.e., linked, to the 3' end of component c) Component e): at least one effector gene whose transcription is activated by the expression product of component b) binding to component c); to its preparation and its use; to vectors comprising the nucleic acid construct, cells comprising these vectors, and to the use of the nucleic acid construct for the preparation of a medicament.
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