发明名称 |
A DOMINANT SELECTABLE MARKER FOR GENE TRANSFORMATION AND DISRUPTION IN YEASTS |
摘要 |
The present invention provides a novel dominant selectable marker system in yeast that is based on an aminoglycoside, nourseothricin (NST). This compound possesses a powerful antifungal activity against <i>Candida albicans</i> and <i>S. cerevisiae</i>. The invention provides a cognate drug resistance marker for use in gene transformation and disruption experimentation in <i>Candida albicans</i> and <i>Saccharomyces cerevisiae</i>. In particular, the invention presents: 1) direct utility for gene manipulations in both clinically and experimentally relevant strains regardless of genotype and without affecting growth rate, or hyphal formation; and 2) applicability to antifungal drug discovery, including target validation and various forms of drug screening assays.
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申请公布号 |
WO0161019(A3) |
申请公布日期 |
2002.09.26 |
申请号 |
WO2001CA00194 |
申请日期 |
2001.02.19 |
申请人 |
MCGILL UNIVERSITY;ROEMER, TERRY;BUSSEY, HOWARD;DAVISON, JOHN |
发明人 |
ROEMER, TERRY;BUSSEY, HOWARD;DAVISON, JOHN |
分类号 |
A61K38/00;C07K14/40;C12N9/10;C12N15/81;(IPC1-7):C12N15/81 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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