| 摘要 |
The present invention relates to a screening, diagnostic and/or dosage method of a known or unknown agonist (full agonist or partial agonist) and/or a known or unknown antagonist (neutral antagonist or inverse agonist) for a calcium-coupled receptor, comprising following steps:incubating one or more cells expressing apoaequorin and said calcium-coupled receptor with "coelenterazine i" having the formula as depicted in Figure 3 or a derivative thereof, in order to reconstitute active aequorin by said cells; contacting said cell(s) with the agonist and/or antagonist of said receptor on a solid support, and,measuring light emitted by said cell(s), allowing the screening, diagnosis and/or dosage of said agonist and/or antagonist of said receptor, and, possibly, recovering said agonist or antagonist of said receptor from the reaction mixture. The present invention also relates to a corresponding device allowing the performance of said method and to the agonist and/or antagonist of said calcium-coupled receptor identified by said method and device. |
