发明名称 |
METHOD FOR IMPROVING DETECTION SENSITIVITY OF PATHOGEN OR NUCLEIC ACID |
摘要 |
PROBLEM TO BE SOLVED: To provide a method for testing a pathogen or a nucleic acid with a higher sensitivity than that of a nested PCR method. SOLUTION: This method for improving the detection sensitivity for the pathogen or nucleic acid is characterized by carrying out a PCR method, especially a two-stage PCR method such as the nested PCR method and then performing a Southern blot technique. The concentration of 10<-8> pg/μl can be detected by combining the PCR method, especially the nested PCR method with the Southern blot technique and a remarkably high sensitivity as compared with single use of each technique can be obtained. |
申请公布号 |
JP2002272499(A) |
申请公布日期 |
2002.09.24 |
申请号 |
JP20010082617 |
申请日期 |
2001.03.22 |
申请人 |
UNIV NIHON |
发明人 |
MAEDA TAKAHIDE;NAKAMURA HITOSHI |
分类号 |
G01N33/50;C12N15/09;C12Q1/68 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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