THREE DIMENSIONAL BIOCHIP

摘要

A biochip is formed with a plurality of optically clear hydrogel cells attached to the top surface of a solid substrate in the form of an array. Each of the cells is formed of a hydrogel of polyethylene glycol, polypropylene glycol or a copolymer thereof having reactive isocyanate groups. Nonhybridization binding entities are immobilized in these cells, which entities are effective to selectively sequester a target protein or other comparable biomolecule. Different binding entities are immobilized in different cells to create a biochip that can be used to assay for a number of target biomolecules.