发明名称 |
NUCLEOTIDES LABELLED WITH AN INFRARED DYE AND THEIR USE IN NUCLEIC ACID DETECTION |
摘要 |
Nucleoside-5'-triphosphates and phosphoramidites which carry a residue absorbing in the long wavelength region, preferably a carbocyanine group of the general formula (I), on the base portion or on the phosphorus atom (see formula I) in which R1 and R2 each denote hydrogen or together form a phenyl residue; R3 denotes hydrogen if linkage with the nucleotide is via the R4 position or it denotes a -NHCS- group if linkage with the nucleotide is via the R3 position; both R4 and R5, or R5, alone denote an alkylsulfonyl group with n being a number from 3 to 5 or R4 represents a -NHCS- group with n being a number from 3 to 8, as well as the use of the compounds to label, detect and sequence nucleic acids.
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申请公布号 |
CA2145405(C) |
申请公布日期 |
2002.09.17 |
申请号 |
CA19942145405 |
申请日期 |
1994.07.30 |
申请人 |
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发明人 |
MUEHLEGGER, KLAUS;HOELTKE, HANS-JOACHIM;BIRKNER, CHRISTIAN;VON DER ELTZ, HERBERT;MIDDENDORF, LYLE R. |
分类号 |
G01N33/533;C07F9/572;C07H19/04;C07H19/10;C07H19/20;C07H21/00;C12N15/09;C12Q1/68;G01N21/78;G01N33/50;G01N33/566;G01N33/58;(IPC1-7):C12Q1/68;C09B23/12 |
主分类号 |
G01N33/533 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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