METHOD FOR PRODUCING MONOCLONAL ANTIBODY

摘要

PROBLEM TO BE SOLVED: To provide a low cost, efficient and in vitro method for producing a monoclonal antibody having a high specificity and high affinity. SOLUTION: This method for producing the monoclonal antibody is provided by (a) co-expressing an antibody gene with a gene encoding a PPIase having a chaperone-like activity in a transformed body, and producing the monoclonal antibody as a soluble fraction, or (b) solubilizing the monoclonal antibody expressed as an inclusion body with a modifying agent, and further folding it in the presence of the PPIase having the chaperone-like activity.