发明名称 |
METHOD FOR REACTIVATING IMMOBILIZED UROKINASE COLUMN |
摘要 |
PURPOSE: A method for reactivating an immobilized urokinase column is provided, thereby recycling immobilized urokinase column to obtain a target protein and consequently reducing costs therefor. CONSTITUTION: The method for reactivating a urokinase immobilized column comprises the steps of: passing a fusion protein through a urokinase covalently bound column to cut the fusion protein; inserting a urokinase specific mutagen into the fusion protein passed through the urokinase immobilized column to unfold a protein structure; and removing the urokinase specific mutagen from the column to refold the urokinase, in which the mutagen is guanidine HCl, urea and ion type surfactant; the column is filled with a resin consisting of sepharose gel; and the mutagen is removed by inserting a buffer into the column.
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申请公布号 |
KR20020072349(A) |
申请公布日期 |
2002.09.14 |
申请号 |
KR20010012177 |
申请日期 |
2001.03.09 |
申请人 |
KOREA GREEN CROSS CORPORATION;LEE, EUN KYU |
发明人 |
LEE, EUN KYU |
分类号 |
C12P21/00;(IPC1-7):C12P21/00 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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