METHOD FOR COUNTING PHOTONS IN LASER-SCANNING SYSTEMS

摘要

The invention relates to a method for counting photons in the detection channel of a laser-scanning assembly, preferably a laser-scanning microscope. According to said method, the amplitude of the incoming photons is determined using several thresholds and the threshold determination is coupled to a time-resolved measurement, a respective pulse being counted for individual thresholds and the sum of the counted pulses being determined for the thresholds. For expediency, when a higher threshold is reached, the counted value for the lower threshold is saved for the count.