<p>The identification of pre-defined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. The exponential, analog nature of the polymerase chain reaction is transformed into a linear, digital signal suitable for this purpose. Single molecules can be isolated by dilution and individual amplified; each product is then separately analyzed for the presence of mutations. The process provides a reliable and quantitative measure of the proportion of variant sequences within a DNA example.</p>
申请公布号
WO0109386(A3)
申请公布日期
2002.09.12
申请号
WO2000US20740
申请日期
2000.07.31
申请人
THE JOHNS HOPKINS UNIVERSITY;KINZLER, KENNETH, W.;VOGELSTEIN, BERT