METHOD FOR THE DETECTION OF NUCLEIC ACID MOLECULES

摘要

A method is disclosed for the simultaneous detection of at least two mutually different nucleic acid molecules in a sample. In a first step a multiplex PCR and in a second step a hybridisation reaction are carried out on immobilised probes in a microarray. The hybridised PCR products are then detected and optionally quantified, whereby the probes applied for the hybridisation reaction, which each hybridise specifically with nucleic acids which are different from each other, have melting points which differ from each other by at most 2 DEG C, and preferably 1 DEG C.